Recombinant:Article Title: STAG2-truncating variants reveal a mosaic STAG2-inactivation pattern and compensatory mechanisms involving cohesin complex remodeling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SMC3 Wendt lab Zuin et al., 201457 Rabbit polyclonal anti-STAG2 Bethyl labs Cat# A302-580A, RRID:AB_2034860 Goat polyclonal anti-STAG1 Abcam Cat# AB4457, RRID:AB_2286589 Goat polyclonal anti-STAG1 Bethyl labs Cat# A300-156A, RRID:AB_185512 Rabbit polyclonal anti-STAG3 Abcam Cat# AB185109 Rabbit polyclonal anti-STAG3 Sigma-Aldrich Cat# SAB4500153, RRID:AB_10745135 Rabbit polyclonal anti-STAG3 Rolf Jessberger – Mouse monoclonal anti-Phospho-Histone H2A.X (Ser139) Thermo Fisher Scientific Cat# 14-9865-80, RRID:AB_2573047 Mouse monoclonal anti-vinculin Santa Cruz Biotechnology Cat# sc-59803, RRID:AB_794011 Rabbit polyclonal anti-HP1β Abcam Cat# ab10478, RRID:AB_297216 Mouse monoclonal anti-β-tubulin Sigma-Aldrich Cat# T8328, RRID:AB_1844090 Biological samples Patient samples Contributing clinicians Table 1 Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat#A11138-03;CAS:53-79-2 Doxycycline Sigma-Aldrich D9891-5G Propidium Iodide Invitrogen Cat# 15541957; CAS: 25535-16-4 Critical commercial assays X-tremeGENETM HP Roche 6366244001 Cell Counting Kit - 8 Sigma-Aldrich 96992 ReliaPrep RNA Cell Miniprep system Promega Z6011 LipofectamineTM 2000 Thermo Fisher Scientific 11668019 NEXTFlex ChIP-seq kit BioOScientific NOVA-5143-01 Deposited data Raw and analyzed data This paper SRA: PRJNA1199213 Experimental models: Cell lines STAG3-EGFP-HCT116 This paper N/A .. HeLa ATCC ATCC® CCL-2, RRID: CVCL_0030 Oligonucleotides siRNA: – – Control non-targeting control sense CGUACGCGGAAUACUUCGAtt This paper N/A Control non-targeting control antisense UCGAAGUAUUCCGCGUACGtt This paper N/A STAG2 sense AGCACUAACAGAUAGG CAAGAGAGT This paper N/A STAG2 antisense ACUCUCUUGCCU AUCUGUUAGUGCUUC This paper N/A Recombinant DNA pRTS1 Bornkamm et al.58 N/A (Continued on next page) iScience 28, 114195, December 19, 2025 e1 iScience Article ll OPEN ACCESS
Cell Counting:Article Title: STAG2-truncating variants reveal a mosaic STAG2-inactivation pattern and compensatory mechanisms involving cohesin complex remodeling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SMC3 Wendt lab Zuin et al., 201457 Rabbit polyclonal anti-STAG2 Bethyl labs Cat# A302-580A, RRID:AB_2034860 Goat polyclonal anti-STAG1 Abcam Cat# AB4457, RRID:AB_2286589 Goat polyclonal anti-STAG1 Bethyl labs Cat# A300-156A, RRID:AB_185512 Rabbit polyclonal anti-STAG3 Abcam Cat# AB185109 Rabbit polyclonal anti-STAG3 Sigma-Aldrich Cat# SAB4500153, RRID:AB_10745135 Rabbit polyclonal anti-STAG3 Rolf Jessberger – Mouse monoclonal anti-Phospho-Histone H2A.X (Ser139) Thermo Fisher Scientific Cat# 14-9865-80, RRID:AB_2573047 Mouse monoclonal anti-vinculin Santa Cruz Biotechnology Cat# sc-59803, RRID:AB_794011 Rabbit polyclonal anti-HP1β Abcam Cat# ab10478, RRID:AB_297216 Mouse monoclonal anti-β-tubulin Sigma-Aldrich Cat# T8328, RRID:AB_1844090 Biological samples Patient samples Contributing clinicians Table 1 Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat#A11138-03;CAS:53-79-2 Doxycycline Sigma-Aldrich D9891-5G Propidium Iodide Invitrogen Cat# 15541957; CAS: 25535-16-4 Critical commercial assays X-tremeGENETM HP Roche 6366244001 Cell Counting Kit - 8 Sigma-Aldrich 96992 ReliaPrep RNA Cell Miniprep system Promega Z6011 LipofectamineTM 2000 Thermo Fisher Scientific 11668019 NEXTFlex ChIP-seq kit BioOScientific NOVA-5143-01 Deposited data Raw and analyzed data This paper SRA: PRJNA1199213 Experimental models: Cell lines STAG3-EGFP-HCT116 This paper N/A .. HeLa ATCC ATCC® CCL-2, RRID: CVCL_0030 Oligonucleotides siRNA: – – Control non-targeting control sense CGUACGCGGAAUACUUCGAtt This paper N/A Control non-targeting control antisense UCGAAGUAUUCCGCGUACGtt This paper N/A STAG2 sense AGCACUAACAGAUAGG CAAGAGAGT This paper N/A STAG2 antisense ACUCUCUUGCCU AUCUGUUAGUGCUUC This paper N/A Recombinant DNA pRTS1 Bornkamm et al.58 N/A (Continued on next page) iScience 28, 114195, December 19, 2025 e1 iScience Article ll OPEN ACCESS
Chromatin Immunoprecipitation:Article Title: STAG2-truncating variants reveal a mosaic STAG2-inactivation pattern and compensatory mechanisms involving cohesin complex remodeling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SMC3 Wendt lab Zuin et al., 201457 Rabbit polyclonal anti-STAG2 Bethyl labs Cat# A302-580A, RRID:AB_2034860 Goat polyclonal anti-STAG1 Abcam Cat# AB4457, RRID:AB_2286589 Goat polyclonal anti-STAG1 Bethyl labs Cat# A300-156A, RRID:AB_185512 Rabbit polyclonal anti-STAG3 Abcam Cat# AB185109 Rabbit polyclonal anti-STAG3 Sigma-Aldrich Cat# SAB4500153, RRID:AB_10745135 Rabbit polyclonal anti-STAG3 Rolf Jessberger – Mouse monoclonal anti-Phospho-Histone H2A.X (Ser139) Thermo Fisher Scientific Cat# 14-9865-80, RRID:AB_2573047 Mouse monoclonal anti-vinculin Santa Cruz Biotechnology Cat# sc-59803, RRID:AB_794011 Rabbit polyclonal anti-HP1β Abcam Cat# ab10478, RRID:AB_297216 Mouse monoclonal anti-β-tubulin Sigma-Aldrich Cat# T8328, RRID:AB_1844090 Biological samples Patient samples Contributing clinicians Table 1 Chemicals, peptides, and recombinant proteins Puromycin Thermo Fisher Scientific Cat#A11138-03;CAS:53-79-2 Doxycycline Sigma-Aldrich D9891-5G Propidium Iodide Invitrogen Cat# 15541957; CAS: 25535-16-4 Critical commercial assays X-tremeGENETM HP Roche 6366244001 Cell Counting Kit - 8 Sigma-Aldrich 96992 ReliaPrep RNA Cell Miniprep system Promega Z6011 LipofectamineTM 2000 Thermo Fisher Scientific 11668019 NEXTFlex ChIP-seq kit BioOScientific NOVA-5143-01 Deposited data Raw and analyzed data This paper SRA: PRJNA1199213 Experimental models: Cell lines STAG3-EGFP-HCT116 This paper N/A .. HeLa ATCC ATCC® CCL-2, RRID: CVCL_0030 Oligonucleotides siRNA: – – Control non-targeting control sense CGUACGCGGAAUACUUCGAtt This paper N/A Control non-targeting control antisense UCGAAGUAUUCCGCGUACGtt This paper N/A STAG2 sense AGCACUAACAGAUAGG CAAGAGAGT This paper N/A STAG2 antisense ACUCUCUUGCCU AUCUGUUAGUGCUUC This paper N/A Recombinant DNA pRTS1 Bornkamm et al.58 N/A (Continued on next page) iScience 28, 114195, December 19, 2025 e1 iScience Article ll OPEN ACCESS
|
Bethyl is a verified supplier 